Plant Biotechnology Team

The team conducts research in the field of biopharming and bioenergetics with the use of plant genetic engineering methods and in vitro cultures. In the first area, research is focused on the production of Virus-Like Particles (VLPs) in plant expression systems as components of oral and injection vaccines, carriers for epitopes of various antigens and constituents of bionanoparticles.

Research profile

• Production of antigenic proteins in plant expression systems.
• Plant material processing toward oral and injection formulas of vaccines.
• Development of effective methodology of oral and injection-oral immunisation.
• Utilisation of VLPs as carriers for immunogenic epitopes and bionanoparticles constituents.
• Recognition of the molecular and physiological basis of Miscanthus tolerance to abiotic stresses, including low temperatures and soil contamination with heavy metals.
• Development of methods of transformation and regeneration of Miscanthus.
• Obtaining of transgenic Miscanthus with altered biomass properties.
• The use of miscanthus biomass for production of bioethanol, biogas, biocharcoal and biocomposities.
• Micropropagation of Miscanthus via somatic embryogenesis from generative and vegetative tissues.

Methods

• DNA in vitro recombination techniques,
• plant transformationusing Agrobacterium andbiolistic method,
• VLPs production using transient expression systems
• VLPs purification and analysis
• investigations of immune response
• plant in vitro cultures, somatic embryogenesis
• analysis of (trans)gene expression: qPCR, ddPCR, Western Blot, ELISA, immunohistochemistry
• complex analysis of reaction of energy grasses to stresses, including morphology (biometric traits), anatomy (histology and histochemistry), physiology  (RuBisCo activity assays, photosynthetic gas exchange, chlorophyll fluorescence, electrolyte leakage), biochemistry (contents of metabolites) and molecular biology (RNAseq, qPCR, ddPCR )
• analysis of lignocellulosic complex: FTIR, immunohistochemistry, gas chromatography
• fementation and carbonisation of plant biomass

Current research activities

• Production of VLPs assembled by HBV antigens and their derivatives in transgenic and transplastomic plants and in transient expression systems.
• Processing of plant material containing VLPs toward oral and injection formulas of vaccines.
• Development of effective immunisation via injection-oral route using purified VLPs and lyophilised plant tissue.
• Studies of Miscanthus tolerance to cold stress and soil contamination with heavy metals.
• Development of efficient method of Miscanthus somatic embryogenesis from generative and vegetative tissues.
• Verification of Miscanthus transformation and regeneration methods for practical applications.
• The use of Miscanthus biomass for production of bioethanol, biogas, biocharcoal and biocomposities.
• Modification of cell wall composition and structure of model plants.

Selected papers

• Pyrski M., Mieloch A.A.,Plewiński A., Basińska-Barczak A., Gryciuk A., Bociąg P., Murias M., Rybka J.D., Pniewski T. (2019). Parenteral-oral immunization with plant-derived HBcAg as a potential therapeutic vaccine against chronic hepatitis B. Vaccines 7:211.
• Pniewski T., Milczarek M., Wojas-Turek J., Pajtasz-Piasecka E., Wietrzyk J., Czyż M. (2018). Plant lyophilisate as an oral booster vaccine against HBV. Vaccine 36:6070-6076.
• Cerazy-Waliszewska J., Jeżowski S., Łysakowski P., Waliszewska B., Zborowska M., Sobańska K., Ślusarkiewicz-Jarzina A., Białas W., Pniewski T. (2019). Potential of bioethanol production from biomass of various Miscanthus genotypes in three-year plantations in west-central Poland. Industrial Crops and Products 141:111790.
• Ślusarkiewicz-Jarzina A. , Ponitka A., Cerazy-Waliszewska J., Wojciechowicz M.K., Sobańska K., Jeżowski S., Pniewski T. (2017). Effective and simple in vitro regeneration system of Miscanthus sinensis, M. × giganteus and M. sacchariflorus for planting and biotechnology purposes. Biomass and Bioenergy 107:219-226.

Grants