Cereal Phenomics Team

 
Head
Post-doctoral degree Anetta Kuczyńska
 
Staff
Krzysztof Mikołajczak, PhD

Piotr Ogrodowicz, PhD
Sławomir Franaszek, PhD Eng.
Michał Kempa, MSc (PhD student)
Martyna M. Bodzak, MSc (PhD student)
Renata Trzeciak, MSc Eng.
Alina Anioła
Renata Holewińska

 

The team performs quantitative genetics and genomics research on barley, wheat and legumes, dealing with phenotypic observations of genome manifestations at the cellular and whole plant level. The conducted research focuses on the application of modern genomics techniques in key research areas, as well as on understanding the genetic and molecular mechanisms controlling the phenome of cereal plants and their responses to stress factors (abiotic and biotic). These analyzes are supported by new generation phenotyping that enables non-invasive imaging of the growth dynamics of aboveground and underground parts of plants.

 
 
Research profile
  • Genetic maps and QTLs localisation.
  • Pleiotropic effects of semi-dwarf genes in barley.
  • Genetic background of seed technological quality in wheat.
  • Transcriptomic, proteomic and metabolomic analyses.
  • Development of method of rapid homozygotisation of cereal and legume hybrids.

 

Methods
  • in vitro cultures,
  • molecular analyses,
  • field and glasshouse experiments,
  • next generation phenotyping and genotyping
  • biometrical and statistical methods.

 

Current research activities
  • Genetic maps and  localisation of QTLs determining barley resistance to water deficit.
  • Phenotypic effects of the sdw1/denso locus in spring barley (Hordeum vulgare L.).
  • Pleiotropic effects of genes involved in photoperiodic reaction in relation to susceptibility to fusarium ear blight and accumulation of mycotoxins in spring barley.
  • The impact of abiotic stresses on the variability of the barley lipidome and phenome in relation to the expression of the LTP2 gene.
  • Explanation of the hormone crosstalk and its role in shaping the plant architecture in barley (Hordeum vulgare L.)
  • Effects of drought on the development and architecture of barley root system
  • Genetic and molecular background of variability of grain technological traits in wheat (Triticum aestivum L.).
  • Development of biotechnological methods for extending and acceleration of legume breeding.

 

Selected papers
  • Mikołajczak K., Ogrodowicz P., Ćwiek-Kupczyńska H., Weigelt-Fischer K., Mothukuri S.R., Junker A., Altmann J., Krystkowiak K., Adamski T., Surma M., Kuczyńska A., Krajewski P. (2020). Image phenotyping of spring barley (Hordeum vulgare L.) RIL population under drought: selection of traits and biological interpretation. Frontiers in Plant Science 11: 743.
  • Ogrodowicz P., Kuczyńska A., Mikołajczak K., Adamski T., Surma M., Krajewski P., Ćwiek-Kupczyńska H., Kempa M., Rokicki M., Jasińska D. (2020). Mapping of quantitative trait loci for traits linked to fusarium head blight in barley. PLOS ONE 15(2): e0222375.
  • Rodziewicz P., Chmielewska K., Sawikowska A., Marczak Ł., Łuczak M., Bednarek P., Mikołajczak K., Ogrodowicz P., Kuczyńska A., Krajewski P., Stobiecki M. (2019). Identification of drought responsive proteins and related proteomic QTLs in barley. Journal of Experimental Botany 70: 2823-2837.
  • Surma M., Kuczyńska A., Mikołajczak K., Ogrodowicz P., Adamski T., Ćwiek-Kupczyńska H., Sawikowska A., Pecio A., Wach D., Józefaciuk G., Łukowska M., Zych J., Krajewski P. (2019). Barley varieties in semi-controlled and natural conditions - response to water shortage and changing environment. Journal of Agronomy and Crop Science 205: 295-308.
  • Janiak A., Kwasniewski M., Sowa M., Kuczyńska A., Mikołajczak K., Ogrodowicz P., Szarejko I. (2019). Insights into barley root transcriptome under mild drought stress with an emphasis on gene expression regulatory mechanisms. International Journal of Molecular Sciences 20: 6139.
  • Piasecka A., Sawikowska A., Kuczyńska A., Ogrodowicz P., Mikołajczak K., Krystkowiak K., Gudyś K., Guzy-Wróbelska J., Krajewski P., Kachlicki P. (2017). Drought related secondary metabolites of barley (Hordeum vulgare L.) leaves and their mQTLs. The Plant Journal 89(5): 898-913.
 
Research projects
 
Project no: 2016/22/D/NZ9/0042
Project title: HvGAMYB transcription factor in flowering time regulation and its association with photoperiod response under drought conditions in spring barley (Hordeum vulgare L.)
Project leader: P. Ogrodowicz
TYPE: SONATA 12
Duration: 10th August 2017 – 9th February 2022

Project no: 2016/22/D/NZ9/0043
Project title: Modulation of genome-wide genes expression of barley flag leaf via combined abiotic stresses
Project leader: K. Mikołajczak
TYPE: SONATA 12
Duration: 10th August 2017 – 9th February 2022
 
Project no: 2016/23/B/NZ9/03548
Project title: Explanation of the hormone crosstalk and its role in shaping the plant architecture in barley (Hordeum vulgare L.)
Project leader: A. Kuczyńska
TYPE: OPUS 12
Duration: 6th September 2017 – 5th September 2022
 
Project no: 2019/35/B/NZ9/00208
Project title: Melatonin as a pivotal mediator for shaping root architecture and drought adaptation via modulation of phytohormones crosstalk in barley (Hordeum vulgare L.)
Project leader: A. Kuczyńska
TYPE: OPUS 18
Duration: 27th July 2020 – 16th July 2023
 
Project no: ID: 206
Project title: The architecture and development of the roots system in barley plants grown under drought conditions in relation to flowering acceleration
Project leader: P. Ogrodowicz
TYPE: European Plant Phenotyping Network 2020, 5th Call TA
Duration: 12th March 2020 – 31st October 2023
 
Project no: ID: 426
Project title: High-throughput phenotyping of barley varieties response to drought for understanding crops adaptation to arid climate
Project leader: K. Mikołajczak
TYPE: European Plant Phenotyping Network 2020, 5th Call TA
Duration: 12th March 2020 – 31st October 2023
 
Project no: Task 14
Project title: Association studies and molecular determinants of spring barley resistance to environmental stresses
Project leader: A. Kuczyńska
TYPE: Ministry of Agriculture and Rural Development, Biological progress
Duration: 1st January 2021 – 31st December 2025