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Cereal Phenotyping and Genotyping Team
Na skróty

Cereal Phenotyping and Genotyping Team

Leader

Assoc. Prof Anetta Kuczyńska

Staff

Prof. Tadeusz Adamski
Prof. Maria Surma
Krzysztof Mikołajczak, PhD
Piotr Ogrodowicz, PhD
Michał Kempa, MSc (PhD student)
Kamil Kowalik, MSc Eng. (PhD student)
Renata Trzeciak, MSc Eng.
Alina Anioła
Renata Holewińska

The team performs quantitative genetics and genomics research on barley, wheat and legumes. Obtained in various ways populations of homozygous lines are phenotyped and genotyped using next generation high-throughput methods. The aim of the study is to search for relations between variability at the phenotypic and molecular level. Agricultural traits linked with yield quality and quantity as well as resistance to biotic and abiotic stresses are investigated.
Research profile
  • Genetic maps and QTLs localisation.
  • Pleiotropic effects of semi-dwarf genes in barley.
  • Genetic background of seed technological quality in wheat.
  • Transcriptomic, proteomic and metabolomic analyses.
  • Development of method of rapid homozygotisation of cereal and legume hybrids.
Methods:
  • in vitro cultures,
  • molecular analyses,
  • field and glasshouse experiments,
  • next generation phenotyping and genotyping
  • biometrical and statistical methods.
Current research activities
  • Genetic maps and  localisation of QTLs determining barley resistance to water deficit.
  • Phenotypic effects of the sdw1/denso locus in spring barley (Hordeum vulgare L.).
  • Pleiotropic effects of genes involved in photoperiodic reaction in relation to susceptibility to fusarium ear blight and accumulation of mycotoxins in spring barley.
  • The impact of abiotic stresses on the variability of the barley lipidome and phenome in relation to the expression of the LTP2 gene.
  • Explanation of the hormone crosstalk and its role in shaping the plant architecture in barley (Hordeum vulgare L.)
  • Effects of drought on the development and architecture of barley root system
  • Genetic and molecular background of variability of grain technological traits in wheat (Triticum aestivum L.).
  • Development of biotechnological methods for extending and acceleration of legume breeding.
Selected papers
  • Chmielewska K., Rodziewicz P., Swarcewicz B., Sawikowska A., Krajewski P., Marczak Ł., Ciesiołka D., Kuczyńska A., Mikołajczak K., Ogrodowicz P., Krystkowiak K., Surma M., Adamski T., Bednarek P., Stobiecki M. (2016). Analysis of drought induced proteomic and metabolomic changes in barley (Hordeum vulgareL.) leaves and root unravels some aspects of biochemical mechanisms involved in drought tolerance. Frontiers in Plant Science 7: 1108. DOI: 10.3389/fpls.2016.01108
  • Mikołajczak K., Ogrodowicz P., Gudyś K., Krystkowiak K., Sawikowska A., Frohmberg W., Górny A., Kędziora A., Jankowiak J., Józefczyk D., Karg G., Andrusiak J., Krajewski P., Szarejko I., Surma M., Adamski T., Guzy-Wróbelska J., Kuczyńska A. (2016). Quantitative trait loci for yield and yield-related traits in spring barley populations derived from crosses between European and Syrian cultivars. PlosOne 11(5): e0155938, DOI: 10.1371/journal.pone.0155938
  • Kuczyńska A., Surma M., Adamski T., Mikołajczak K., Krystkowiak K., Ogrodowicz P. (2013). Effects of the semi-dwarfing sdw1/denso gene in barley. Journal of Applied Genetics 54(4): 381-390. DOI: 10.1007/s13353-013-0165-x
 
Research projects
  • Project no: 2016/22/D/NZ9/0042
    Project title: HvGAMYB transcription factor in flowering time regulation and its association with photoperiod response under drought conditions in spring barley (Hordeum vulgare L.)
    Project leader: P. Ogrodowicz
    TYPE: SONATA
    Duration: 10th August 2017 – 9th August 2020
  • Project no: 2016/22/D/NZ9/0043
    Project title: Modulation of genome-wide genes expression of barley flag leaf via combined abiotic stresses
    Project leader: K. Mikołajczak
    TYPE: SONATA
    Duration: 10th August 2017 – 9th August 2020
  • Project no: 2016/23/B/NZ9/03548
    Project title: Explanation of the hormone crosstalk and its role in shaping the plant architecture in barley (Hordeum vulgare L.)
    Project leader: A. Kuczyńska
    TYPE: OPUS
    Duration: 3rd September 2017 – 2nd September 2020
  • Project title: Increasing of domestic feed protein usage for the production of high quality animal products in conditions of sustainable development

    Research area: New methods and techniques for improving the value of legume varieties
    Coordinator in IPG PAS: W. Święcicki

    Task 2.3: Biotechnological tools for the acceleration of biological progress in grain legumes breeding
    Project leader: M. Surma

    Task 2.4: Distant crosses within the Lupinus, Pisum and Vicia - searching for new genetic variability and a method to shorten the breeding cycle by in vitro cultures

    Project leader: A. Kuczyńska
    TYPE: Government Long-term Program
    Duration: 1st January 2016 – 31st December 2020
    www.bialkoroslinne.iung.pl

  • Project no: 2015/17/B/NZ9/01481
    Project title:Effects of abiotic stresses on the LTP2 gene expression level in relation to lipidome and phenome in barley (Hordeum vulgare L.)
    Project leader: A. Kuczyńska
    TYPE: OPUS
    Duration: 20th January 2016 – 19th January 2019
  • Project no: HOR hn-801-8/14 zad. 3
    Project title: The effect of 1B/1R translocation on effectiveness of wheat DH lines development and technological quality
    Project leader: T. Adamski
    TYPE: Ministry of Agriculture and Rural Development, Biological progress
    Duration: 1st January 2014 – 31st December 2020