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Plant Molecular Physiology and Cytogenetics Team
Na skróty

Plant Molecular Physiology and Cytogenetics Team

Leader

Assoc. Prof. Arkadiusz Kosmala

Staff

Prof. Zbigniew Zwierzykowski
Danuta Babula-Skowrońska, PhD
Tomasz Książczyk, PhD
Izabela Pawłowicz, PhD
Dawid Perlikowski, PhD
Adam Augustyniak, MSc (PhD student)

Joanna Filder, MSc (PhD student)
Joanna Majka, MSc (PhD student)
Katarzyna Masajada, MSc (PhD student)

Włodzimierz Zwierzykowski, Msc. Eng.

The research is focused on the forage grasses from the Lolium-Festuca complex. Lolium and Festuca species encompasses numerous complementary traits. L. multiflorum (Italian ryegrass) and L. perenne (perennial ryegrass) are the species with a high forage quality but rather low tolerance to abiotic and biotic stresses. Otherwise, F. pratensis (meadow fescue) and F. arundinacea (tall fescue) are characterized by a high level of resistance to pathogens (e.g. Microdochium nivale), winter-hardiness, and tolerance to frost, drought and salinity but they cannot compete with Lolium species with reference to productivity and quality in comfortable environmental conditions. Both Festuca species and their amphiploid and introgression derivatives are regarded as models among forage grasses for the research associated with mechanisms of tolerance to environmental stresses, mainly - drought (F. arundinacea) and low temperature (F. pratensis). These plant materials are also extremely interesting objects for cytogenetic studies focused on plant chromosome organization and evolution.

In parallel, the analysis on gene interaction network associated with the activity of ABI1 protein phosphatase in winter oilseed rape (Brassica napus var. oleifera) under environmental stress conditions, is performed. ABI1 protein phosphatase (group A PP2C) is a central negative regulator of the ABA-mediated signaling pathway and it is involved in a reversible phosphorylation of many proteins. The involvement of ABI1 in a control of transcription factor HB6-dependent gene network under salt and drought stresses, is analyzed

Research profile

  • Molecular basis of plant tolerance to abiotic stresses (low temperature, drought, and salinity) and resistance to pathogens,
  • Organization and evolution of plant chromosome- and genome-specific sequences,
  • Transfer of genes for environmental stress tolerance from Festuca species into Lolium species,
  • Molecular and physiological mechanisms of stress response plasticity in poliploid species.

Methods:

  • analysis of gene expression at transcript (real time RT-PCR ) and protein level (2-D electrophoresis, mass spectrometry, and Western blot),
  • analysis of root system architecture under water deficit conditions,
  • physiological analyses (WC, RWC, electrolyte leakage, gas exchange parameters, and chlorophyll fluorescence),
  • analysis of cellular antioxidant system efficiency under environmental stresses,
  • tests for plant tolerance to low temperature and drought in natural and simulated conditions,
  • fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH),
  • analysis of primary metabolites and membrane lipids accumulation,
  • analysis of protein-protein interactions (yeast two-hybrid system, pull-down, BiFC)
  • generation of transgenic plants with gene over-expression and gene modified functions using CRISPR/Cas9 system.

Selected papers

  • Perlikowski D., Kierszniowska S., Sawikowska A., Krajewski P., Rapacz M., Eckhardt Ä., Kosmala A.(2016). Remodeling of leaf cellular glycerolipid composition under drought and re-hydration conditions in grasses from the Lolium-Festuca complex. Frontiers in Plant Science,7:1027 DOI: 10.3389/fpls.2016.01027
  • Perlikowski D., Czyżniejewski M., Marczak Ł., Augustyniak A., Kosmala A. (2016). Water deficit affects primary metabolism differently in two Lolium multiflorum/Festuca arundinacea introgression forms with a distinct capacity for photosynthesis and membrane regeneration. Frontiers in Plant Science, 7:1063 DOI: 10.3389/fpls.2016.01063
  • Project no: 2017/25/N/NZ9/00001
    Project title: Analysis of molecular mechanisms of frost tolerance in Lolium multiflorum/Festuca arundinacea introgression forms
    Project leader: A. Augustyniak

Research projects

  • Project no: 2017/25/N/NZ9/00001
    Project title: Analysis of molecular mechanisms of frost tolerance in Lolium multiflorum/Festuca arundinacea introgression forms
    Project leader: A. Augustyniak

      TYPE: PRELUDIUM
      Duration: 1st February 2018 – 31st January 2020

     Project leader: D. Babula-Skowrońska
     TYPE: OPUS
    
Duration:  26th July 2017 – 25th July 2020
 
  • Project no: 2016/23/B/N29/00820
    Project title: Insight into molecular mechanisms of tolerance to water deficit and capacity to regenerate after stress cessation in the selected species and hybrids of Lolium-Festuca forage grasses
    Project leader: A. Kosmala
      TYPE: OPUS
     
Duration:  3rd July 2017 – 2nd July 2020
 
  • Project no: HOR hn-801-8/14 - 35
    Project title: Identification of genes associated with the expression of winter-hardiness and drought tolerance in the Lolium multiflorum/Festuca arundinacea introgression forms
    Project leader: A. Kosmala
     TYPE: Ministry of Agriculture and Rural Development, Biological progress
     Duration: 1st January 2014 – 31st December 2020