Regulation of Gene Expression Team
Agnieszka Kiełbowicz-Matuk, PhD
Anna Kasprzewska, PhD
The research is focused on two Solanum species, S. tuberosum (cultivated species) and S. sogarandinum (wild species), possessing various cold acclimation capacity, and nine genotypes within Hordeum vulgare differing in water stress tolerance.
- Molecular basis of plant tolerance to abiotic stresses (low temperature, drought, salinity).
- Isolation and identification of those genes which activity leads to plant acclimation to low temperature and adaptation to drought.
- Identification of those genes which expression is associated with the maintenance of yield parameters upon stress conditions.
- Functional analysis of the proteins encoded by isolated genes in development and acclimation to stress.
- cultivation of plant material in vitro and in vivo in a phytotron,
- tests of plant tolerance to low temperature and drought in simulated conditions,
- physiological analyses (RWC, electrolyte leakage, gas exchange parameters),
- analysis of genes expression at the transcript (RT-PCR, Northern blot) and protein level (Western blot),
- isolation of the transcription factors binding to the cis-regulatory elements present in the promoter regions of the genes (Gold Yeast One-Hybrid System, Y1H; Electrophoretic Mobility Shift Assay, EMSA),
- study of protein-protein interactions (Co-immunoprecipitation, Co-IP; Gold Yeast Two-Hybrid System, Y2H).
Current research activities
The main research is associated with investigating molecular mechanisms responisble for the regulation of Solanum SsBBX24 gene expression, that encodes the protein containing two zinc finger B-box domains, in the circadian cycle under development and stress conditions leading to cell dehydration. This research is conducted in the frame of Institute's statutory research task. The main aspects of the study include: (i) identification of transcription factors that regulate the SsBBX24 gene expression at different phases of the circadian cycle under normal and stress conditions using the Gold Yeast One-Hybrid System (Y1H) and Electrophoretic Mobility Shift Assay method (EMSA); (ii) identification of proteins or protein complexes that interact with the SsBBX24 protein at different phases of the circadian rhythm under normal growth and stress conditions by means of the Gold Yeast Two-Hybrid System (Y2H) and Co-immunoprecipitation (Co-IP) method.
- Kiełbowicz-Matuk A., Czarnecka J., Banachowicz E., Rey P., Rorat T. (2017). Solanum tuberosum ZPR1 encodes a light-regulated nuclear DNA-binding protein adjusting the circadian expression of StBBX24 to light cycle. Plant, Cell & Environment 40(3): 424–440, DOI:0.1111/pce.12875
- Talar U., Kiełbowicz-Matuk A., Czarnecka J., Rorat T.(2017) Genome-wide survey of B-box proteins in potato (Solanum tuberosum) - identification, characterization and expression patterns during diurnal cycle, etiolation and deetiolation. Plos ONE, DOI 10.1371/journal.pone.0177471.
- Majka J., Książczyk T., Kiełbowicz-Matuk A., Kopecky D., Kosmala A. (2017). Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping. Plos ONE. 12 (6): e0179043.. DOI: https://doi.org/10.1371/journal.pone.0179043
- Kiełbowicz-Matuk A., Banachowicz E., Turska-Tarska A., Rey P., Rorat T. (2016). Expression and characterization of a barley phosphatidylinositoltransfer protein structurally homologous to the yeast Sec14p protein. Plant Science 246: 98-111. DOI: 10.1016/j.plantsci.2016.02.0
National Science Centre
Project number: 2014/15/B/NZ9/04809
Title: Functional analysis of the SsBX24 protein containg zinc binding domains in circadian clock during development and response to salinity
Principal investigator: T. Rorat/ A. Kiełbowicz-Matuk
Duration: 20 Jully 2015 - 19 July 2018