Regulation of Gene Expression Team
Anna Kasprzewska, PhD
Klaudia Grądzka, MSc. (scholarship winner)
The research is focused on Solanum species, S. tuberosum (cultivated species) and S. sogarandinum (wild species), possessing various cold acclimation capacity, as well as the wild and cultivated tomato species Solanum pennelli and Solanum lycopersicum.
- Identification of transcriptional regulators among B-box zinc finger domain (StBBX) proteins that control the expression of direct inducers of flowering and tuberization in cultivated potato,
- Identification of transcription factors that regulate the expression of StBBX genes under circadian clock control,
- Investigating the function of StBBX proteins in growth and developmental processes in cultivated potato regulated by light and the circadian clock, as well as in response to abiotic stresses.
- cultivation of plant material in vitro and in vivo in a phytotron,
- analysis of genes expression at the transcript (RT-qPCR, Northern blot) and protein level (Western blot),
- isolation of the transcription factors binding to the cis-regulatory elements present in the promoter regions of the genes (Gold Yeast One-Hybrid System, Y1H; Electrophoretic Mobility Shift Assay, EMSA),
- study of protein-protein interactions (Co-immunoprecipitation, Co-IP; Gold Yeast Two-Hybrid System, Y2H),
- transformation of Solanum plants using A. tumefaciens (overexpression of selected genes in plants and obtaining knockout mutants for selected genes using CRISPR/Cas9 techniques).
Current research activities
The research of the group is related to the understanding of mechanisms of adaptation of Solanum tubersoum species to changing environmental conditions during vegetative and generative development. The team are essentially focused on understanding the biological function of B-box zinc finger (BBX) proteins and the regulators of their expression in growth and development processes controlled by light and the circadian clock under conditions of optimal growth and in response to stress factors. The main aspects of the study include: (i) identification of transcription factors that regulate the BBX genes expression at different phases of the circadian cycle under normal and stress conditions using the Gold Yeast One-Hybrid System (Y1H) and Electrophoretic Mobility Shift Assay method (EMSA); (ii) identification of proteins or protein complexes that interact with the BBX proteins at different phases of the circadian rhythm under normal growth and stress conditions by means of the Gold Yeast Two-Hybrid System (Y2H) and Co-immunoprecipitation (Co-IP) method.
- Talar U., Kiełbowicz‐Matuk A. (2021) Beyond Arabidopsis: BBX Regulators in Crop Plants. International Journal of Molecular Sciences 22: 2906. DOI: 10.3390/ijms22062906.
- Grądzka K., Kiełbowicz-Matuk A. (2020). Białka B-Box u roślin – heterogeniczność strukturalna i funkcjonalna. Postępy Biologii Komórki. Tom 47, nr 3: 225–246.
- Parrotta L., Aloisia I., Suannoa C., Faleri C., Kiełbowicz-Matuk A., Bini L., Cai G., Del Duca S. (2019) A low molecular-weight cyclophilin localizes in different cell compartments of Pyrus communis pollen and is released in vitro under Ca2+ depletion. Plant Physiology and Biochemistry 144: 197-206.
- Kiełbowicz-Matuk A., Czarnecka J., Banachowicz E., Rey P., Rorat T. (2017). Solanum tuberosum ZPR1 encodes a light-regulated nuclear DNA-binding protein adjusting the circadian expression of StBBX24 to light cycle. Plant, Cell & Environment 40(3): 424-440, DOI:0.1111/pce.12875.
- Talar U., Kiełbowicz-Matuk A., Czarnecka J., Rorat T.(2017) Genome-wide survey of B-box proteins in potato (Solanum tuberosum) - identification, characterization and expression patterns during diurnal cycle, etiolation and deetiolation. Plos ONE, DOI 10.1371/journal.pone.0177471.
- Majka J., Książczyk T., Kiełbowicz-Matuk A., Kopecky D., Kosmala A. (2017). Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping. Plos ONE. 12 (6): e0179043. DOI: https://doi.org/10.1371/journal.pone.0179043.
GrantsNational Science CentreProject number: 2014/15/B/NZ9/04809Title: Functional analysis of the SsBX24 protein containg zinc binding domains in circadian clock during development and response to salinityPodtype: OPUSPrincipal investigator: T. Rorat/ A. Kiełbowicz-MatukDuration: 20 Jully 2015 - 19 July 2018