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Legume Comparative Genomics Team
Na skróty

Legume Comparative Genomics Team

Leader

Prof. Wojciech K. Święcicki

Staff

Andrzej Górny, PhD
Dominika Ratajczak, MSc Eng.
Paulina Wilczura, MSc
Czesława Nawrot, Eng.
Katarzyna Beczek
 

Legume Comparative Genomics Team works on phenotype and molecular characteristics of Lupinus, Pisum and Lathyrus genetic resources, genetic background of selected agricultural traits of lupins and pea, physiological traits connected with genotype-environment interaction in narrow-leafed lupin and pea as well as transcriptome profiling of some agricultural traits in Lupinus genus. The aim of research is to determine dependence of seed yield quantity and quality from physiological processes, then to find possibilities to improve resistance of cultivars to pathogens in diverse environmental conditions.

Research profile

  • Preservation of genetic resources and analysis of the phenotypic and molecular variability of Lathyrus, Lupinus and Pisum genera
  • Localisation of new genes and genetic map construction for Lathyrus, Lupinus and Pisum genera
  • Identification of genes involved in agricultural traits formation in lupins on the basis of RNA-seq analysis
  • Expression analysis of genes involved in oligosaccharide biosynthesis pathway and resistance to ascochyta blight in pea as well as alkaloid biosynthesis and resistance to Fusarium wilt in lupins
  • Characterisation and mapping of quantitative traits loci connected with nitrogen usage in pea
  • Identification of physiological-morphological, biochemical and genetic factors underlying relationship between legume seed yield quality and level and leaf photosynthesis process, root development and nitrogen fixation, in various environmental conditions (drought, nitrogen and phosphorus deficit, chilling)
  • Analysis of variability and inheritance of traits involved in physiological processes in narrow-leafed lupin and pea.Evaluation of genotype and environment influence on selected physiological indexes
  • Analysis of total content and quality composition of alkaloids and oligosaccharides in seeds

 

Research methods

  • genetic mapping with the use of molecular markers
  • quantitative trait mapping
  • gene expression analysis
  • gas chromatography for determination of antinutrient content
  • the use of mobile devices for efficiency measurements of plant physiological processes connected with yielding

 

Current works

  • Mapping of new genes in Pisum
  • Identification of genes involved in alkaloid synthesis pathway in white and yellow lupin and yellow lupin resistance to Fusarium wilt with the use of RNA-seq as well as expression analysis of selected genes
  • Expression profiling of genes of metabolic pathways connected with oligosaccharide content in seeds and activated by response to  Didymella pinodes attack in pea
  • Interspecific variation analysis in selected genera of Leguminosae family
  • Analysis of genotype-environment interaction for traits connected with efficiency of physiological processes in Pisum and Lupinus
  • Relationship between yield and quality of seeds and leaf photosynthesis processes, root development and nitrogen fixation in different environmental conditions in pea
  • Valorisation of gene resources in collections of Lathyrus, Lupinus and Pisum genera

 

Pubications

  • Górynowicz B., Święcicki W., Pilarczyk W., Mikulski W. (2018). Correlation of seed yield and its components and chlorophyll fluorescence parameters in the narrow leafed lupin (Lupinus angustifolius L.). Breeding Grasses and Protein Crops in the Era of Genomics Eds. Brazauskas G., Statkeviciute G., Jonaviciene K. Springer, EUCARPIA Switzerland: 191-195
  • Kroc M., Rybiński W., Wilczura P., Kamel K., Kaczmarek Z., Barzyk P., Święcicki W. (2017). Quantitative and qualitative analysis of alkaloids composition in the seeds of a white lupin (Lupinus albus L.) collection. Genetic Resources and Crop Evolution  64(8): 1853-1860
  • Gawłowska M., Święcicki W., Lahuta L., Kaczmarek Z. (2017). Raffinose family oligosaccharides in seeds of Pisum wild taxa, type lines for seed genes, domesticated and advanced breeding materials. Genetic Resources and Crop Evolution 64: 569–578
  • Salmanowicz B.P., Langner M., Mrugalska B., Ratajczak D., Górny A.G. (2017). Grain quality characteristics and dough rheological properties in Langdon durum-wild emmer wheat chromosome substitution lines under nitrogen and water deficits. Journal of the Science of Food and Agriculture 97(7): 2030-2041
  • Wnuk A., Gozdowski D., Górny A., Wyszyński Z., Kozak M. (2017). Data visualization in yield component analysis: an expert study. Scientia Agricola 74(2): 118-126
  • Grela R.E., Kiczorowska B., Samolińska W., Matras J., Kiczorowski P., Rybiński W., Honczakowska E. (2017). Chemical composition of leguminous seeds: part I- content of basic nutrients, amino acids, phytochemical compounds, and antioxidant activity. European Food Research Technology. DOI 10.1007/s00217-017-2849-7

 

Research projects

  • Project No: 2014/13/N/NZ9/03943
    Project title: Expression profiles of selected genes determining quinolizidine alkaloids biosynthesis in various organs of the narrow-leafed lupin (Lupinus angustifolius L.)
    PI: K. Czepiel
    Type: PRELUDIUM
    Duration: 27 stycznia 2015 - 27 stycznia 2019
  • Project No: PBS3/A8/28/2015
    Project title: SEGENMAS New generation sequencing and association mapping as methods for generating molecular markers of agricultural traits in narrow-leafed lupin
    Co-ordinator: B. Wolko
    Type: PBS NCBR
    Consortium members: 7
    Duration: March 1, 2015 – February 28, 2018
  • Project No: HOR hn-801-8/14 task 40
    Project title: Identification of pea genome regions responsible for selected parameters of physiological effectiveness as an important element of abiotic stress response.
    PI: W. Święcicki
    Type: MRiRW Postęp biologiczny
    Duration: January 1, 2014 – December 31, 2020
  • Project No: HOR hn-801-8/14 task 41
    Project title: Identification and mode of inheritance of genes responsible for fungi disease resistance and low alkaloid content in quality improvement of lupins, particularly yellow lupin
    PI: W. Święcicki
    Type: MRiRW Postęp biologiczny
    Duration: January 1, 2014 – December 31, 2020
  • Project No: HOR hn-801-8/14 zad. 42
    Project title: Genetic variation analysis and pyramidizing of genes controlling agricultural traits of white lupin
    PI: W. Rybiński
    Type: MRiRW Postęp biologiczny
    Duration: January 1, 2014 – December 31, 2020
  • Project No: RM 222/2015 (decision of the Council of Ministers)
    Project title: Increased usage of domestic protein sources for production of high quality animal products under sustainable conditions
Consortium: 18 members
Area realised in IPG PAS: Novel methods and techniques for quality improvement of legume cultivars
PI: W. Święcicki
Type:  Long-standing Governmental Program 2016-2020
Funding body: Ministry of Agriculture and Rural Development
Duration: January 1, 2016 – December 31, 2020
www.bialkoroslinne.iung.pl
Task 2.1. Improvement of the usefulness of pea (Pisum sativum L.), narrow-leafed lupin (Lupinus angustifolius L.) and white lupin (L. albus L.) by reducing the anti-nutritional content of raffinose oligosaccharides in seeds and reducing the susceptibility of pea to ascochyta blight. M. Gawłowska
Task 2.2. Identification of genes conditioning the content of alkaloids and the formation and maintenance of generative organs in lupins. M. Kroc
Task 2.5. Analysis of variability and mode of inheritance of physiological indicators in narrow-leaved lupin and pea and the possibility of their use in improving plant productivity. B. Górynowicz
Task 2.6. Photosynthesis of leaves, formation and abortion of generative organs, development of roots and atmospheric nitrogen fixation as processes important for the level and quality of legume yield under stress conditions. A. Górny
  • Project No: IHAR RM 104/2015 (decision of the Council of Ministers)
Project title: Creation of biological progress scientific bases and plant gene resources protection as sources of innovation and support for sustainable agriculture and national food safety
Area realised in IPG PAS (Agreement No 8/PW 1.2 2016): Domestic collection of genetic resources of marginal grain legumes
PI: W. Rybiński
Type: Long-standing Governmental Program2016-2020
Funding body: Ministry of Agriculture and Rural Development
Duration: December 1, 2015 – November 30, 2020