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Cereal Genomics Team

Cereal Genomics Team

Leader

Prof. Halina Wiśniewska

Staff

Maciej Majka, MSc (PhD student)
Jolanta Belter, MSc Eng

The team conducts research on theoretical and practical aspects of interspecific and intergeneric hybrids (substitution and translocation lines) within Triticum, X Triticale and Secale. The main goal is to extend the genetic diversity of those species and to transfer new, valuable traits to improve the quality of yield. This research has become more important taking into consideration rapid break of resistance to biotic and abiotic stresses.

Research profile

  • Cytogenetic analyses of secondary wheat-rye forms with the introgression of diploid wheat (Triticum monococcum) chromatin.
  • Investigation of controlled introgression of resistance genes against biotic stresses (pathogenic fungi) to wheat and triticale from Aegilops and Agropyron.
  • Molecular identification of resistance genes to fungal diseases using verified markers.
  • Search, creation, assessment and collection of head blight resistance sources in wheat and triticale.
  • Studies on identification and translocation of eyespot (caused by Oculimacula yallundae and O. acuformis) resistance genes to cultivated wheat.

Methods:

  • molecular cytogenetics (GISH, multi-colour GISH, FISH, BAC?FISH),
  • resistance genes identification using molecular and izoenzymatic markers,
  • in vitro tissue cultures (hybrids stabilization),
  • inoculation tests in field conditions and assesment of examined genotypes using phenotypic markers.

Current research activities

  • Study of susceptibility to leaf rust (Puccinia triticina) and variability of qualitative traits in triticale introgresive lines with introgresive fragments of Triticum monococcum (2x) and hexaploid wheat genome.
  • Chromosome identification in diploid and tetraploid goatgrasses (Aegilops spp.) and Triticeae hybrids using cytogenetic markers.
  • Search, creation, assessment and collection of head blight resistance sources in wheat.
  • Study on triticale genotypes resistance to Fusarium head blight and mycotoxin accumulation in kernels.
  • Identification and translocation of eyespot resistance genes to cultivated wheat.

Selected papers

  • Góral T., Wiśniewska H., Ochodzki P., Walentyn-Góral D., Kwiatek M. (2012). Reaction of winter triticale breeding lines to Fusarium head blight and accumulation of Fusarium metabolites in grain in two environments under drought conditions. Cereal Res. Commun. 41(11): 106 -115.
  • Kwiatek M., Majka M., Wiśniewska H., Apolinarska B., Belter J. (2014). Effective transfer of chromosomes carrying leaf rust resistance genes from Aegilops tauschii Coss. into hexaploid triticale (X Triticosecale Witt.) using Ae. tauschii × Secale cereale amphiploid forms. J Appl Genetics DOI 10.1007/s13353-014-0264-3
  • Kwiatek M., Błaszczyk L., Wiśniewska H., Apolinarska B. (2012). Aegilops-Secale amphiploids: chromosome categorisation, pollen viability and identification of fungal disease resistance genes. J. Appl. Genet. 53: 37-40.
  • Książczyk T., Apolinarska B., Kulak-Książczyk S., Wiśniewska H., Stojałowski S., Łapiński M. (2011). Identification of the chromosome complement and the spontaneous 1R/1V translocations in allotetraploid Secale cereale × Dasypyrum villosum hybrids through cytogenetic approaches. J. Appl. Genet. 52: 305-391.
  • Warzecha T., Adamski T., Kaczmarek Z., Surma M., Chełkowski J., Wiśniewska H. Krystkowiak K., Kuczyńska A. (2011). Genotype ? by-environment interaction of barley DH lines infected with Fusarium culmorum (W.G.Sm.) Sacc. Field Crop Res. 120: 21-30.
  • Apolinarska B., Wiśniewska H., Wojciechowska B. (2010). Aegilops-rye amphiploids and substitution rye used for introgression of genetic material into rye. J. Appl. Genet. (4): 413-429.
  • Wiśniewska H., Basiński T., Chełkowski J., Perkowski J. (2008). Accumulation of type a trichothecenes in a bioassay inoculated with Fusarium sporotrichiodes. Cereal Res. Commun. 36B: 409-411.

Grants