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Gene Structure and Function Team
Na skróty

Gene Structure and Function Team

Leader

Prof. Barbara Naganowska

Staff

Prof.  Bogdan Wolko
Michał Książkiewicz, PhD
Karolina Susek, PhD
Wojciech Bielski, MSc (PhD student)
Piotr Plewiński, MSc (PhD student)
Elżbieta Rudy, MSc (PhD student)
Sandra Rychel, MSc (PhD student)
Waldemar Ulaszewski, MSc (PhD student)
Magdalena Tomaszewska, MSc

The research is focused on genomics and molecular biology of lupins. The model species for this genus is the narrow-leafed lupin (Lupinus angustifolius L.). The research includes generating of molecular markers, genetic and physical mapping of the narrow-leafed lupin genome as well as identification of genes connected with expression of important desirable traits and analysis of their structure and function. The work concerns in particular genes participating in the process of flowering induction, in symbiotic nitrogen fixation, specific for legumes, in synthesis of phenylpropanoid pathway enzymes and fatty acids, and genes conferring resistance of the narrow-leafed lupin to pathogenic fungi. White lupin (L. albus L.) is also the object of research comprising genotyping by sequencing, transcriptome analysis and genetic mapping. Cytogenetic mapping of Lupinus spp. genomes is aimed at construction of an integrated map of the narrow-leafed lupingenome and at analysis of evolutionary chromosome rearrangements within the genus. Comparative analyses of the Lupinus sp. genome with sequences of other Fabaceae genomes (Medicago sativa, Lotus japonicus, Glycine max, Cajanus cajan, Phaseolus vulgaris) available in the databases are also conducted, together with identification of syntenic regions.

 

Research profile

  • structure and organization analysis of the narrow-leafed lupin genome regions including genes conferring important desirable traits,
  • molecular phylogeny of selected Lupinus representatives, evaluation of synteny within the genus as well as between lupins and other legumes,
  • cytogenetic insight into genome structure of the narrow-leafed, yellow and white lupin,
  • genetic variation analysis of white lupin,
  • generation of molecular markers linked with agricultural traits and estimation of their usefulness for selection of genotypes in breeding programmes.

Methods:

  • selection of BAC clones from the nuclear genome library of Lupinus angustifolius by hybridization of radioactively labeled probes specific for selected genes with library macroarrays,
  • restriction ?fingerprinting? of BAC clone inserts and contig construction,
  • functional annotation of BAC clone sequences with the use of available sequence data of lupins and other legume plants (model species Medicago truncatula and Lotus japonicus as well as Glycine max) ? gene searching,
  • generation of sequence-defined molecular markers of different types based on BAC clone sequences of the narrow-leafed lupin and on sequences of other related species available in the databases,
  • introduction of the genetic markers on the genetic map ? genetic mapping with the use of computer software,
  • cytogenetic mapping of BAC clones in metaphase chromosomes by the technique of fluorescence in situ hybridization (BAC-FISH),
  • genotyping by sequencing (GBS).

Current research activities

  • Comparative mapping by FISH with chromosome-specific markers (BAC clones) from the genomic DNA library of the reference species (L. angustifolius) as molecular probes. Insight into the evolution of lupin genomes at chromosome level.
  • Sequence analysis of selected genes and their genetic and physical location in the nuclear genome of the narrow-leafed lupin (Lupinus angustifolius L.). The genes of interest are: 7 genes encoding enzymes connected with the process of symbiotic nitrogen fixation and nodule development, 4 genes encoding subunits of acetyl-coenzyme A, the key enzyme of fatty acids synthesis in plants, 3 genes encoding enzymes of phenylpropanoid synthesis pathway and 8 genes conferring resistance   to pathogens, characterized earlier.
  • Determination of genome location and nucleotide sequence of flowering genes homologues, especially the FT group genes of crucial role in the initiation of flowering process in plants. Delivery of technology for monitoring narrow-leafed lupin genotypes with molecular markers based on the sequence of flowering induction gene.

Selected papers

 

Grants

LEGATO  Legumes for the agriculture of tomorrow

Project type: Large Collaborative Project (EU FP7-KBBE-2013-7-single stage)

Duration: 1 January 2014 ? 31 December 2017

Co-ordinator: Richard Thomas, INRA, Francja

IPG PAS co-ordinator: Bogdan Wolko

Consortium: 29 members

 

Project no: PBS 224227

Project title: New generation sequencing and association mapping as methods for generating molecular markers of agricultural traits in narrow-leafed lupin SEGENMAS

Principal investigator: B. Wolko

Duration: 1 March 2015 - 31 August 2018


Project no: Biological Progress Task 39/2014

Project title: Flowering induction trait in white lupin and yellow lupin ?genetic and molecular background

Principal investigator: B. Wolko/ M. Książkiewicz

Duration: 1 January 2014 -31 December 2020