Na skróty

Plant Microbiome Structure and Function Team

Lider:

Assc. Prof. Lidia Błaszczyk

 

Staff:

Prof. Jerzy Chełkowski (retired professor)

Sylwia Salamon, PhD

Aneta Basińska-Barczak MSc Eng. (PhD student)

Katarzyna Mikołajczak MSc Eng. (PhD student)

 

Research is focused on knowing the microbiome of the selected cereal species and determining the ecological role of microorganisms associated with the rhizosphere, phylosphere and endosphere of these plants. Microorganisms play a major role in natural ecosystems and in modern agriculture as a result of their nutritional versatility and various interactions with plants. Microorganisms are important decomposers and recyclers of organic materials. They interact with plant roots in the rhizosphere or with above-ground plant components; while living in close association with plants, they are located either outside or within plant tissues. Therefore, the main topic of the Team is to broaden the understanding of the selected cereal species microbiome dynamics and its impact on the growth and fitness of plants.

 

Research profile:

a) assessment of diversity and composition of microorganisms communities associated with rhizosphere, phyllosphere and endosphere of plants

b) recognition of the transmission mode of endogenous microorganisms

c) determining the impact of the host genotype, host organs, and plant growth conditions on the compositions and distribution of microorganisms in rhizosphere, phyllosphere and endosphere of plants

d) defining the morphological, anatomical, physiological, and molecular wheat plants’ reactions to changes in their microbiome

 

Methods:

  • isolation of microorganisms from the rhizosphere, phyllosphere and endosphere
  • culture of microorganisms on solid and liquid substrates
  • molecular identification of microorganisms based on the next-generation sequencing and Sanger sequencing
  • morphological and anatomical analysis of plants using the light microscopy, scanning electron microscope (SEM), transmission electron microscopy (TEM) and confocal microscopy
  • quantitative RT-PCR expression analysis of selected plant genes
  • plant proteome and metabolome analysis using complementary mass spectrometric and chromatographic methods

 

Selected papers:

  • Gromadzka K., Błaszczyk L., Chełkowski J., Waśkiewicz A. (2019). Occurrence of Mycotoxigenic Fusarium Species and Competitive Fungi on Preharvest Maize Ear Rot in Poland. Toxins., 11:224, doi.org/10.3390/toxins11040224
  • Stępień Ł., Gromadzka K., Chełkowski J., Basińska-Barczak A., Lalak-Kańczugowska J. (2019). Diversity and mycotoxin production by Fusarium temperatum and Fusarium subglutinans as causal agents of pre-harvest Fusarium maize ear rot in Poland. J. Appl. Genetics, 60:113-121.
  • Marecik R., Błaszczyk L., Biegańska-Marecik R., Piotrowska-Cyplik A. (2018). Screening and identification of Trichoderma strains isolated from natural habitats with potential to cellulose and xylan degrading enzymes production. Pol. J. Microbiol., 2:181-190.
  • Błaszczyk L., Basińska A., Ćwiek H, Gromadzka K, Popiel D., Stępień Ł. (2017). Suppressive effect of Trichoderma on toxigenic Fusarium species. Pol. J. Microbiol., 1:85-100.
  • Gromadzka K., Wit M., Górna K., Chełkowski J., Waśkiewicz A., Ochodzki P., Warzecha R. (2017). Cereal Res. Commun., 45: 93-103.
  • Błaszczyk L., Strakowska J., Chełkowski J., Gąbka-Buszek A., Kaczmarek J. (2016). Trichoderma species occurring on wood with decay symptoms in mountain forests in Central Europe: genetic and enzymatic characterization. J. Appl. Genet., 57-397-407.
  • Najda A., Błaszczyk L., Winiarczyk K., Dyduch J., TchórzewskaD. (2016). Comparative studies of nutritional and health-enhancing propertiesin the “garlic-like” plant Allium ampeloprasum var. ampeloprasum (GHG-L) and A. sativum. Sci. Hort., 201:247-255
  • Tchórzewska D., Deryło K., Błaszczyk L., Winiarczyk K. (2015). Tubulin cytoskeleton during microsporogenesis in the male-sterile genotype of Allium sativum and fertile Allium ampeloprasum L. Plant Reprod., 28:171-182.

 

Grants:

  • Project no: 2017/27/B/NZ9/01591
Project title: The common wheat (Triticum aestivum L.) endosphere mycobiome dynamics and its impact on the growth and fitness of plant
Subtype: OPUS 14
Principal investigator:  L. Błaszczyk
Duration: 29 July 2018 - 28 July 2021
 
  • Project no: 2016/19/B/NZ9/03083
Project title: The molecular basis of wheat responses (Triticum aestivum L.) to Trichoderma spp. root colonization
Subtype: OPUS 10
Principal investigator:  L. Błaszczyk
Duration: 23 July 2016 - 22 July 2020
 
  • Project no: 2016/19/N/NZ9/01625
Project title: Analysis of changes occurring in the roots of wheat (Triticum aestivum L.) as a result of the interaction of these plants with the fungi Trichoderma.
Subtype: PRELUDIUM 10
Principal investigator:  A Basińska-Barczak
Duration: 23 July 2016 - 22 December 2018