Scientific achievements

Achievements 2009-2013

• Identification in the  Lupinus angustifolius genome two gene-rich regions, pointing out synteny with duplicated regions of the Glycine max. genome which is reflected in identical gene order and orientation.
  
  Książkiewicz M., Wyrwa K., Szczepaniak A., Rychel S., Majcherkiewicz K., Przysiecka Ł., Karlowski W., Wolko B., Naganowska B. (2013). Comparative genomics of Lupinus angustifolius gene-rich regions: BAC library exploration, genetic mapping and cytogenetics. BMC Genomics 14: 79.

• Method of quantitative DNA detection (Real Time PCR, SybrGreen) of the Alternaria fungi that may replace time-consuming microscope monitoring. Much higher allergeny of Alternaria and Cladosporium spores than presented in literature required to reveal symptoms was observed.
  
  Kasprzyk I., Sulborska A., Nowak M., Szymańska A., Kaczmarek J., Haratym W., Weryszko-Chmielewska E., Jędryczka M. (2013). Fluctuation range of the concentration of airborne Alternaria conidiospores sampled at different geographical locations in Poland (2010?2011). Acta Agrobotanica 66: 65?76.

• Proteins differing in the level of accumulation in rye lines of various growth type are associated with the presence or absence of a semi-dwarf sdw1 gene. To these proteins belong: protein encoded by the ES2A gene of giberelino-dependent expression, thermal shock proteins (HSP) and large alpha and beta subunits of RuBisCo.
  
  Kuczyńska A., Kosmala A., Surma M., Adamski T. (2012). Identification of tillering node proteins differentially accumulated in barley recombinant inbred lines with different juvenile growth habits. Int. J. Mol. Sci.13: 10410-10423.

• The intensity of photosynthesis in drought conditions of Lolium multiflorum/Festuca arundinacea introgressive forms is not dependent on the degree of opening of the stomatal apparatus and activity of photosystemu II, but from the Calvin cycle performance.  Also a positive correlation was found between the intensity of photosynthesis and the level of accumulation of chloroplast aldolase and protein 14-3-3, involved in the regulation of the performance of the regenerative phase of the Calvin cycle.
  
  Perlikowski D., Kosmala A., Rapacz M., Kościelniak J., Pawłowicz I., Zwierzykowski Z. (2013). Influence of short-term drought conditions and subsequent re-watering on the physiology and proteome of Lolium multiflorum/Festuca arundinacea introgression forms with contrasting levels of tolerance to long-term drought. Plant Biology doi: 10.1111/plb.12074.

• Method of molecular detection of Leptosphaeria maculans and L. biglobosa pathogenic fungi, the cause of stem canker of cruciferes on rapeseed, with the use of species-specific fluorescent probes (Real Time PCR). It was used in the decision support system for the protection of rapeseed against stem canker (SPEC).
  
  Kaczmarek J., Jedryczka M., Cools H., Fitt B.D.L., Lucas J.A., Latunde-Dada A.O. (2012). Quantitative PCR analysis of abundance of airborne propagules of Leptosphaeria species in air samples from different regions of Poland. Aerobiologia, 28: 199-212

• The effectiveness of uptake and use of nitrogen and water in the formation of grain weight are characteristics regulated by a complex genetic system. The compexity of their inheritance may significantly limit the progress in breeding of physiologically more effective wheat cultivars better adapted to stress conditions and/ or sustainable agriculture.
  
  Ratajczak D., Górny A.G. (2012). Water- and nitrogen-dependent alterations in the inheritance mode of transpiration efficiency in winter wheat at the leaf and whole-plant level. J. Appl. Genet. 53: 377-388.

• Lines with the recessive allel of the sdw1 gene in the SSD lines population of winter rye have, in addition to the reduced length of the blades, also smaller size of leaf epidermal cells, smaller density of stomatal apparatus per 1mm, smaller size of the vascular bundles as welll as smaller length and width of the leaf. This suggests the pleiotropic effect of sdw1 gene at the level of organs, tissues and cells.
  
  Kuczyńska A., Wyka T. (2011). The effect of the denso dwarfing gene on morpho-anatomical characters in barley recombinant inbred lines. Breeding Sci. 61: 275-280.

• Protocol of data processing and analysis from experiments conducted by means of  chromatin immunoprecipitation and high-throuput DNA sequencing (ChIP-Seq). This method has been applied in the study on genomic location of SEP3 transcription factor binding sites in Arabidopsis thaliana. Thanks to that, its role in the repression of genes governing the flowering time of flowering and in the activation of homeotic genes determining flowering was discovered.
  
  Kaufmann K., Mui?o J.M., Osteras M., Farinelli L., Krajewski P., Angenent G.C. (2010). Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP). Nature Protocols 5 (3): 457-472.
  
  Kaufmann K., Wellmer F., Mui?o J.M., Ferrier T., Wuest S., Kumar V., Serrano-Mislata A., Madue?o F., Krajewski P., Meyerowitz E.M., Angenent G.C., Riechmann J.L. (2010). Orchestration of floral initiation by APETALA1. Science 328: 85-89.

• Różnice w twardości ziarna pszenżyta w znacznym stopniu zależą od składu jakościowo-ilościowego specyficznych białek sekaloindolowych (SIN) występujących we frakcji friabilinowej. Na podstawie profili uzyskanych nowo opracowaną metodą wolnostrefowej elektroforezy kapilarnej (CZE) stwierdzono, że w ziarniakach pszenżyta występują po dwa białka typu SIN-a i SIN-b. Formy alleliczne SIN-a1 i SIN-b1 są obecne w ziarniakach             o niższej twardości, natomiast formy SIN-a2 i SIN-b2 stwierdzano w odmianach o twardszym ziarnie.
  
  Salmanowicz B.P. (2010). Identification and characterization of high molecular weight secalins from triticale seeds by capillary zone electrophoresis. Electrophoresis 31: 2226-2235.

• Twardość ziarna pszenicy zwyczajnej (Triticum aestivum) determinowana jest nie tylko przez geny kodujące białka puroindolinowe Pin-a i Pin-b, zlokalizowane w locus Ha na krótkim ramieniu chromosomu 5D, ale także przez inne rejony genomu. Wykazano, że wpływ warunków środowiska na tę cechę jest mało znaczący, natomiast twardość ziarna istotnie wpływa na cechy warunkujące wartość technologiczną.
  
  Obuchowski W., Salmanowicz B., Banaszak  Z., Adamski T., Surma M., Kaczmarek Z., Majcher M., Ługowska B., Kuczyńska A., Krystkowiak K. (2010).Grain hardness of wheat bred in Poland and its relationship to starch damage during milling. Int. Agrophys. 24: 69-74.

• Infekcja przez chorobotwórczy grzyb Colletotrichum lupini powodujący antraknozę łubinów powoduje znaczne zmiany składu metabolitów wtórnych w liściach roślin łubinu wąskolistnego (Lupinus angustifolius L.). Wykazano zróżnicowanie profili izoflawonoidów      o właściwościach fitoaleksyn (związków wytwarzanych przez rośliny w wyniku infekcji w celu chemicznej obrony przed patogenem) w zależności od wieku liści i sposobu aplikacji zarodników grzyba.
  
  Muth D., Kachlicki P., Krajewski P., Przystalski M., Stobiecki M. (2009). Differential metabolic response of narrow leafed lupine (Lupinus angustifolius) leaves to infection with Colletotrichum lupini. Metabolomics 5: 354-362.

• New classification of  Triticosecale high- weight mass secalin subunits (HMW-SS) by means of high-performance capillary electrophoresis (CZE). Significant variability in qualitatively-quantitative composition of co-existing HMW glutenins of wheat and seclins of rye was found in Triticosecale cornels influencing the technological quality of obtained flour.
  
  Salmanowicz B.P. (2009). CE determination of secaloindoline allelic forms in hexaploid triticale (x Triticosecale Wittmack). J. Sep. Sci. 33: 1-8.
  
  Salmanowicz B.P., Nowak J.(2009). Diversity of monomeric prolamins in triticale cultivars determined by capillary zone electrophoresis. J. Agr. Food Chem. 57:2119-2125.

• Methodology of handling results of multi-characteristic individual experiments and series of experiments using statistical methods based on general mathematical model of observations. It enables obtaing a lot of data on examined genotypes by estimation of multidimensional genetic-breeding parameters of the model, verification of the hyphoteses related with these paratmeters and, first and foremost, selecting the best genotypes in terms of a specific set of features.
  
  Caliński T., Czajka S., Kaczmarek Z., Krajewski P., Pilarczyk W. (2009). Analyzing the genotype-by-environment interactions under a randomization-derived mixed model. Journal of Agricultural, Biological, and Environmental Statistics 14: 224?241.
  
  Caliński T., Czajka S., Kaczmarek Z., Krajewski P., Pilarczyk W. (2009). A mixed model analysis of variance for multi-environment variety trials. Statistical Papers 50: 735?759.

• Evaluation method of wheat resistance to eyespot caused by Cercosporella herpotrichoides fungus based on isoenzymes analysis and with the use of STS Xust SSR2001-7DL marker linked with Pch1 gene determining this characteristic. Selected genotypes were included in the inoculation field tests, which confirmed resistence foreseen by molecular analyses.
  
  Kwiatek M., Pankiewicz K., Korbas M., Wiśniewska H., Danielewicz J. (2012). Identification of Pch1 eyespot resistance gene in the collection of wheat lines (Triticum aestivum L.). J. Plant Prot. Res. 52: 254-258.

• System for Forecasting Disease Epidemics (SPEC) has been introduced to agricultural practice as a joint initiative of Institute of Plant Genetics PAS and DuPont Poland.  Field experiments data shown that fungicide treatments applied in accordance with the SPEC system contributed to the increase in rapeseed yield (0,3 up to 0,6 ton of seeds per hectar). In all cases, the profit associated with the treatment was more than 10-fold above the cost of its implementation.
  
  www.spec.edu.pl