Integrative Plant Biology Team

The Department has been created thanks to the ERA-CHAIR BIO-TALENT The Creation of the Department of Integrative Plant Biology EU FP7 No. 621321 project

Leader: Prof. Robert Malinowski PhD

Professor in the field of natural sciences, in the discipline of biological sciences

Habilitation Adam Mickiewicz University 2012
PhD Warsaw University of Life Sciences 2004 
MSc Warsaw University of Life Sciences 2000

Members:

Previous members:

Research profile

• The role of a vascular tissue in a signal transduction in plants

• Reprogramming of plant development, cell cycle progression and cell differentiation under biotic or abiotic stresses

• Change in assimilate allocation in response to adverse environmental conditions or upon a pathogen attack

• Mechanisms underlying tolerance and resistance to clubroot disease

• Regulation of plant cell and organ size

Research Interests

We are primarily interested in understanding of the developmental plasticity in plants subjected to adverse environmental conditions or pathogen attack.

At present our work is conducted mainly on the interaction between the Plasmodiophora brassicae protist and brassicace plants. Most of the experiments are carried out on the Arabidopsis thaliana - a model plant, but our group works also on the oilseed rape (Brassica napus). Our work also includes the search for molecular mechanisms of the tolerance or resistance. While paying attention to host defence responses to Plasmodiophora brassicae we always try to understand the action of defence genes in a holistic way that includes physiological, cellular and developmental perspective.

Currently our team also works on the developmental plasticity of plant vascular tissues and the role of long-distance transport in adapting plants to abiotic and biotic stress.

Methods and approaches:

The integrative character of our group is further reflected by the fact that we do not restrict ourselves in the choice of experimental methods. Laboratory is fully equipped and allows to perform DNA, RNA, protein and functional experiments as well as various techniques of microscopy to answer basic questions in plant biology.

Our main achievements:

So far our team described cell cycle reprogramming of the host by Plasmodiophora brassicae (Olszak et al., 2019) and provided the evidence for the pathogen-driven mechanisms leading to host carbohydrate redirection during clubroot disease (Walerowski et al., 2018). This work also included the discovery of pathogen-driven increase in the accumulation of SWEET transporters and their role in increasing the availability of sugars to the pathogen. We also described the importance of changes in pectin status for cell enlargement observed during late stages of clubroot disease (Stefanowicz et al., 2021). Recent work of our team aimed at screening of natural genetic variation in Arabidopsis thaliana accessions lead us to the discovery of clubroot resistance gene RPB1 (Ochoa et al., 2023).

In the field of abiotic stress adaptation we found that phloem sap metabolic profile changes can be used as a proxy to understand garden pea response to limited water availability (Blicharz et al., 2021). Changes in C ad N metabolism reflected decrease in cellular growth as well as gas exchange and photosynthesis.

Key words

Arabidopsis thaliana, Plasmodiophora brassicae, plant development, cell growth, cell differentiation, cellular patterning, clubroot, plant vascular systems, plant-microbe interactions

Selected recent publications (See all in Scopus)

• Malinowski R., Singh D., Kasprzewska A., Blicharz S. and Basińska-Barczak A. (2024) Vascular tissue – boon or bane? How pathogens usurp long-distance transport in plants and the defence mechanisms deployed to counteract them. New Phytologist, 243, 2075-2092.

• Blicharz S., Stefanowicz K., Truman, W., Basinska-Barczak A., Singh D., Kasprzewska, A., de Diego, N., Vrobel, O., Cavar Zeljkovic, S., Tarkowski, P. and Malinowski R. (2024) Laser dissection-assisted phloem transcriptomics highlights the metabolic and physiological changes accompanying clubroot disease progression in oilseed rape. The Plant Journal, (in press)

• Ochoa J.C., Mukhopadhyay S., Bieluszewski T., Jędryczka M., Malinowski R. and Truman W. (2023) Natural variation in Arabidopsis responses to Plasmodiophora brassicae reveals an essential role for Resistance to Plasmodiophora brasssicae 1 (RPB1). The Plant Journal, 116, 1421-1440.

• Singh D., Blicharz S., Stefanowicz K., Ragni L., Michalak K., Bagniewska-Zadworna A., and Malinowski R. (2022) Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae. J Vis Exp, e64297.DOI:10.3791/64297-v

• Blicharz S., Beemster GTS, Ragni L., De Diego N., Spichal L., Hernandiz A.E., Marczak L., Olszak M., Perlikowski D., Kosmala A., Malinowski R. (2021) Phloem exudate metabolic content reflects the response to water-deficit stress in pea plants (Pisum sativum L.). The Plant Journal 106 (5): 1338-1355. DOI: https://doi.org/10.1111/tpj.15240 DOI: 10.1111/tpj.15240

• Stefanowicz K., Szymanska-Chargot M., Truman W., Walerowski P., Olszak M., Augustyniak A., Kosmala A., Zdunek A., Malinowski R. (2021) Plasmodiophora brassicae-Triggered Cell Enlargement and Loss of Cellular Integrity in Root Systems Are Mediated by Pectin Demethylation. Frontiers in Plant Science 12. DOI: https://doi.org/10.3389/fpls.2021.711838

• Olszak M., Truman W., Stefanowicz K., Śliwinska E., Ito M., Walerowski P., Rolfe S.,  Malinowski R. (2019). Transcriptional profiling identifies critical steps of cell cycle reprogramming necessary for Plasmodiophora brassicae-driven gall formation in Arabidopsis. The Plant Journal 97 (4):715-729. DOI: https://doi.org/10.1111/tpj.14156

• Walerowski P., Gündel A., Yahaya N., Truman W., Sobczak M., Olszak M., Rolfe S., Borisjuk L., Malinowski R. (2018). Clubroot Disease Stimulates Early Steps of Phloem Differentiation and Recruits SWEET Sucrose Transporters within Developing Galls. The Plant Cell 30 (12), 3058-3073. DOI: https://doi.org/10.1105/tpc.18.00283
  

Projects (finished recently or current)