Multiomics Laboratory

Leader

dr Katarzyna Juszczyk

Members

mgr Aleksandra Burdzińska

Profile

The Laboratory has been established for performing analyses of low molecular weight as well as protein components of plant organisms. It cooperates with several Departments of the Institute and different Polish and foreign scientific institutions. The Laboratory has a gas chromatograph, instruments for High- and Ultra-Performance Liquid Chromatography and Mass Spectrometers. Due to the possibility to hyphenate the standard and nano-flow liquid chromatographs with the high resolution OrbiTrap mass spectrometer it is possible to carry out targeted and untargeted metabolomic and proteomic analyses.

Methods:

• Qualitative and quantitative analysis of lupine alkaloids – GC-FID
• Metabolomic analyses – primary metabolites (amino acids, nucleotides, carbohydrates, lipids), secondary plants metabolites (phenols, polyphenols, flavonoids, terpenes, terpenoids, alkaloids)
  • Analysis of metabolomics with biological activity of crops and medicinal plants
  • Analysis of fungal metabolites
• Lipidiomic: separation and identification of lipids and their derivatives
• Proteomic: separation and identification of proteins and peptides
• Spectrometry analysis of individual compounds or fractions (nanoESI ion source with a built-in autosampler - TriVersa NanoMate, Advion)

Contact: kjus-at-igr.poznan.pl

Key words

Metabolomics, lipidiomics, proteomics, chromatography, mass spectrometry

Instrumentation:

• OrbiTrap high-resolution mass spectrometer (QExactive, Thermo) equipped with HESI-II and nanoESI ion sources
• Nanoflow liquid chromatograph (RSLC nano Ultimate 3000, Dionex, Thermo)
• Ultra-high performance liquid chromatograph with PDA detector (UPLC-PDA, ACQUITY, Waters)
• High performance liquid chromatograph (HPLC 1100, Agilent) with ion trap (IT-MS, Esquire 3000, Bruker)
• NanoESI ion source with built-in autosampler and fraction collector (TriVersa NanoMate, Advion)
• Gas chromatograph with FID detector (GC-2014 Shimadzu)
• Select Series Cyclic Ion-Mobility Separation (IMS) mass spectrometer with Ion sources: ESI, APCI, nanoESI and unispray (Waters Corporation)
• Ultra-high performance liquid chromatograph with PDA Acquity Premier System (Waters Corporation)
• Nano and microflow liquid chromatograph M-class UPLC (Waters Corporation)
• Desorption Electrospray Ionization (DESI XS) for MS imaging (Waters Corporation)
• The APGC source (Waters Corporation) coupled to the Agilent 8890 gas chromatograph (Agilent Technologies)

The integrative multiomics platform based on high-resolution mass spectrometry for metabolome and microbiome research with the use of machine learning"  co-financed by European Union in the program European Funding for Modern Economy (FENG.02.04-IP.04-0025/24-00)